Methotrexate impurity N4 CAS 54643-27-5
| CAS No. | 54643-27-5 |
| Molecular Formula | C7H8N6O2 |
| Molecular Weight | 208.18 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
CATO supplies Methotrexate impurity N4 (CAS 54643-27-5), a pharmacopoeial impurity reference standard (molecular formula C7H8N6O2, MW 208.18 g/mol) used for the identification and quantification of trace impurities in APIs.
Owing to an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage, Methotrexate impurity N4 is typically off-white to pale yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 208.18 g/mol — guide the selection of appropriate sample preparation and analytical techniques. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C7H8N6O2 |
| Molecular Weight | 208.18 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 7.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1972 |
| Nitrogen Atoms | 6 |
Related Methotrexate Standards
- Methotrexate Impurity N5 (CAS 56364-06-8)
- Methotrexate Impurity 60 (CAS CATO-C4X-115460)
- Methotrexate EP Impurity A (CAS 945-24-4)
- Methotrexate Impurity 30 (CAS 881-33-4)
- Methotrexate Impurity N6 (CAS 4261-17-0)
- Methotrexate Impurity N2 (CAS 708-74-7)
- Methotrexate impurity N1 (CAS 4215-07-0)
- Methotrexate Impurity 62 (CAS 942840-87-1)
Recommended Standards
- Ethyl 2-(2-(1H-1,2,4-triazol-5-yl)hydrazono)-2-cyanoacetate (CAS 61033-14-5)
- N-(2-(1H-1,2,4-Triazol-1-yl)ethoxy)-2-amino-2-oxoacetimidoyl cyanide (CAS 89721-56-2)
- Diethyl 2-fluoro-2-(hydroxymethyl)malonate (CAS 756-31-0)
- Dabrafenib Impurity 31 (CAS CATO-C4X-303331)
- Desloratadine Impurity 25 (CAS CATO-C4X-143025)
- Natamycin Impurity 10 (CAS CATO-C4X-204010)
- Trazodone Impurity 37 HCl (CAS CATO-C4X-177237)
- 2-Amino-N-(cyclohexylmethyl)-n,2-dimethylpropanamide (CAS 1251095-18-7)
- Benzyl (2-(2-aminoethoxy)ethyl)carbamate (CAS 1290627-95-0)
- 1-(3-tert-butyl-5-ethynyl-phenyl)hexahydropyrimidine-2,4-dione (CAS 1132942-34-7)
- 1,5-Diphenylpenta-2,4-dien-1-one (CAS 614-57-3)
- 1,2-dimethoxy-3-(prop-1-en-2-yl)benzene (CAS 37388-12-8)
- 2-(2-(6,8-Dichloro-2-methyl-4-oxoquinazolin-3(4H)-yl)acetyl)hydrazinecarbothioamide (CAS 68241-04-3)
- Tau Peptide (301-315) trifluoroacetate salt (CAS 330456-48-9)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
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- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com