Methotrexate Impurity 60 CAS CATO-C4X-115460
| CAS No. | CATO-C4X-115460 |
| Molecular Formula | C8H10N6O2 |
| Molecular Weight | 222.20 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
CATO supplies Methotrexate Impurity 60 (CAS CATO-C4X-115460), a pharmacopoeial impurity reference standard (molecular formula C8H10N6O2, MW 222.20 g/mol) used for the identification and quantification of trace impurities in APIs.
Structurally, Methotrexate Impurity 60 (C8H10N6O2) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. The molecule exhibits a degree of unsaturation (DBE) of 7.0, corresponding to approximately 7 ring(s) or double-bond equivalent(s). With a molecular weight of 222.20 g/mol, it falls into the medium-molecular-weight range. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C8H10N6O2 |
| Molecular Weight | 222.20 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 7.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Nitrogen Atoms | 6 |
Related Methotrexate Standards
- Methotrexate Impurity N5 (CAS 56364-06-8)
- Methotrexate impurity N4 (CAS 54643-27-5)
- Methotrexate EP Impurity A (CAS 945-24-4)
- Methotrexate Impurity 30 (CAS 881-33-4)
- Methotrexate Impurity N6 (CAS 4261-17-0)
- Methotrexate Impurity 63 (CAS 2801801-56-7)
- Methotrexate Impurity 33 (CAS CATO-C4X-115433)
- Methotrexate Impurity 67 (CAS CATO-C4X-115467)
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Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
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- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com