Methotrexate impurity 53 CAS CATO-C4X-115453

Description

Compared with other compounds in its class, the molecular weight of 483.44 g/mol gives Methotrexate impurity 53 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

Methotrexate impurity 53 (CAS CATO-C4X-115453) is a certified pharmaceutical impurity reference standard with the molecular formula C20H21N9O6 and a molecular weight of 483.44 g/mol.

Based on its molecular formula C20H21N9O6, Methotrexate impurity 53 contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 483.44 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.

As a certified reference material (CRM), Methotrexate impurity 53 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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