Methotrexate-d CAS 432545-60-3
| CAS No. | 432545-60-3 |
| Molecular Formula | - |
| Molecular Weight | 485.51 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
Methotrexate-d (C22H23D3N8O5) contains deuterium isotope labeling, a flavonoid core, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
CATO supplies Methotrexate-d (CAS 432545-60-3), a certified reference material (molecular formula C22H23D3N8O5, MW 485.51 g/mol) for analytical method development and quality control.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C22H23D3N8O5 |
| Molecular Weight | 485.51 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 15.5 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate impurity 50 (CAS CATO-C4X-115450)
- Methotrexate impurity 53 (CAS CATO-C4X-115453)
- Methotrexate impurity N3 (CAS 65148-64-3)
- Methotrexate EP Impurity J (CAS 34378-65-9)
- Methotrexate Impurity 61 (CAS 65148-63-2)
- Methotrexate Impurity 18 (CAS CATO-C4X-115418)
- Methotrexate Dimethylamide (CAS 71074-44-7)
- Methotrexate impurity 51 (CAS CATO-C4X-115451)
Recommended Standards
- 1-Benzyl-3-[(4-fluorophenyl)methyl]-5-[(2E)-3-[(4-methoxyphenyl)amino]prop-2-enoyl]-1,2,3,4-tetrahydropyrimidine-2,4-dione (CAS 338399-43-2)
- Nintedanib Impurity 172 (CAS CATO-C4X-1814172)
- Sonidegib (CAS 956697-53-3)
- 7-ACCA Impurity 6 (CAS CATO-C4X-23136)
- Sodium hydroxide (CAS 1310-73-2)
- 2'-Amino-[1,1'-biphenyl]-4-carboxylic acid (CAS 5730-77-8)
- 2-(Ethylthio)-7-methyl-4-phenylpyrazolo[1,5-a][1,3,5]triazine (CAS 908020-59-7)
- 7-Chloro-3,4-dihydro-2H-benzo[b][1,4]thiazine (CAS 106016-84-6)
- 5-(Aminomethyl)-3-cyclopropoxy-N,N-dimethylpicolinamide (CAS 1243471-66-0)
- 4-(Furan-2-yl)-2-methyl-4,5,6,7-tetrahydrothiazolo[5,4-c]pyridine (CAS 1017168-85-2)
- n-(4-Methoxybenzyl)-3-(methylthio)propan-1-amine (CAS 792142-14-4)
- DL-alpha-Tocopherol (CAS 10191-41-0)
- 6-Methyl-2-thioxo-1,2-dihydroquinoline-3-carbaldehyde (CAS 887922-68-1)
- Delta-Sleep Inducing Peptide (CAS 62568-57-4)
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