Estradiol Impurity 27 CAS CATO-C4X-163627

Description

Compared with other compounds in its class, the molecular weight of 284.39 g/mol gives Estradiol Impurity 27 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

Estradiol Impurity 27 (CAS CATO-C4X-163627) is a certified pharmaceutical impurity reference standard with the molecular formula C19H24O2 and a molecular weight of 284.39 g/mol.

The molecular framework of Estradiol Impurity 27 (C19H24O2) is characterized by a steroid core, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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