Methotrexate EP Impurity J CAS 34378-65-9
| CAS No. | 34378-65-9 |
| Molecular Formula | C22H26N8O5 |
| Molecular Weight | 482.49 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Based on its molecular formula C22H26N8O5, Methotrexate EP Impurity J contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 482.49 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
CATO supplies Methotrexate EP Impurity J (CAS 34378-65-9), a pharmacopoeial impurity reference standard (molecular formula C22H26N8O5, MW 482.49 g/mol) used for the identification and quantification of trace impurities in APIs.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
CATO manufactures Methotrexate EP Impurity J under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C22H26N8O5 |
| Molecular Weight | 482.49 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >90% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1966 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate impurity N3 (CAS 65148-64-3)
- Methotrexate Impurity 61 (CAS 65148-63-2)
- Methotrexate impurity 50 (CAS CATO-C4X-115450)
- Methotrexate impurity 53 (CAS CATO-C4X-115453)
- Methotrexate Impurity 18 (CAS CATO-C4X-115418)
- Methotrexate Dimethylamide (CAS 71074-44-7)
- Methotrexate-d (CAS 432545-60-3)
- Methotrexate Impurity 28 (CAS 5939-37-7)
Recommended Standards
- Diundecylsulfane (CAS 35599-82-7)
- Pemetrexed Impurity 54 (CAS 1265908-58-4)
- Copanlisib impurity A (CAS 2132943-82-7)
- Fmoc-β-Ala-Gly-Gly-Gly-OH (CAS CATO-CPTD788410)
- Clofazimine Impurity 13 (CAS CATO-C4X-207113)
- Felodipine Impurity 16 (CAS 52334-51-7)
- Ibrutinib Impurity 28 (CAS CATO-C4X-106428)
- 4-Cyclopropyl-1H-pyrazol-3-amine (CAS 673475-74-6)
- 4-Chloro-N-(1-methoxypropan-2-yl)picolinamide (CAS 1152579-33-3)
- 1,4-Bis((E)-4-bromostyryl)benzene (CAS 58358-55-7)
- (4,6-Dimethylpyrimidin-5-yl)methanol (CAS 1067230-39-0)
- tert-butyl 2-(4-bromo-3,5-dimethyl-1H-pyrazol-1-yl)acetate (CAS 2013373-96-9)
- 3β-DOXYL-5α-cholestane, free radical (CAS 55569-61-4)
- Gastrin Tetrapeptide (CAS 1947-37-1)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com