Cyclophosphamide impurity 01 CAS 35144-64-0
| CAS No. | 35144-64-0 |
| Molecular Formula | C7H15Cl2N2O3P |
| Molecular Weight | 277.09 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The molecular framework of Cyclophosphamide impurity 01 (C7H15Cl2N2O3P) is characterized by an amide group, a phosphonate group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
As a process- or degradation-related impurity, Cyclophosphamide impurity 01 (CAS 35144-64-0, C7H15Cl2N2O3P) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C7H15Cl2N2O3P |
| Molecular Weight | 277.09 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry, keep tightly closed to prevent evaporation |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO, water |
| Stability | Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 0.5 |
| CAS Registry Year (est.) | 1967 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 2 |
Related Cyclophosphamide Standards
- Cyclophosphamide Impurity 9 (CAS 40277-05-2)
- Cyclophosphamide Impurity 12 (CAS 78149-83-4)
- Cyclophosphamide Impurity 19 (CAS 22089-17-4)
- Cyclophosphamide Impurity 22 (CAS 3308-51-8)
- Cyclophosphamide impurity 32 (CAS CATO-C4X-230632)
- 4-Oxo Cyclophosphamide (CAS 27046-19-1)
- Cyclophosphamide impurity 40 (CAS CATO-C4X-230640)
- Cyclophosphamide Impurity 12 (CAS CATO-C4X-230612)
Recommended Standards
- 8-Bromo-1-fluorophenazine (CAS 2379918-33-7)
- Ethyl 2-(4-bromo-3-fluorophenoxy)acetate (CAS 718-13-8)
- 7-Bromo-1-fluorophenazine (CAS 2366994-18-3)
- Ethyl 2-(5-Bromo-2-fluorophenyl)-2-hydroxyacetate (CAS 668969-67-3)
- Torasemide Impurity 13 (CAS CATO-C4X-183613)
- Spiramycin EP Impurity B (CAS CATO-C4X-23252)
- Edaravone Impurity N1 (CAS 6078-46-2)
- 3(2H)-furanone, dihydro-4-(3-methoxyphenyl)- (CAS 907214-31-7)
- N-(Prop-2-yn-1-yl)-2-(trifluoromethyl)pyridin-4-amine (CAS 1857210-16-2)
- tert-Butyl cyclobutyl(3-(1-methylpiperidin-2-yl)pyridin-2-yl)carbamate (CAS 1352490-76-6)
- Orforglipron impurity 21 (CAS CATO-C4X-323421)
- 1,1-Dimethylethyl 4-(3-azetidinylmethyl)-1-piperazinecarboxylate (CAS 1780637-40-2)
- 1-(2-bromo-5-fluorophenyl)propan-2-one (CAS 1250521-52-8)
- D-Glu3-Tirzepatide (CAS CATO-CPTD788927)
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