Methotrexate EP Impurity B CAS 54-62-6
| CAS No. | 54-62-6 |
| Molecular Formula | C19H20N8O5 |
| Molecular Weight | 440.41 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 440.41 g/mol gives Methotrexate EP Impurity B distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
As a process- or degradation-related impurity, Methotrexate EP Impurity B (CAS 54-62-6, C19H20N8O5) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Owing to a flavonoid core, an amide group, a hydroxyl group, an ether linkage, Methotrexate EP Impurity B is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 440.41 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
CATO manufactures Methotrexate EP Impurity B under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C19H20N8O5 |
| Molecular Weight | 440.41 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >90% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 8 |
Related Methotrexate Standards
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate EP Impurity F (CAS 51865-79-3)
- Methotrexate Impurity 64 (CAS 52980-66-2)
- Methotrexate (CAS 59-05-2)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate-d3 (CAS 432545-63-6)
- Methotrexate Impurity 69 (CAS CATO-C4X-115469)
Recommended Standards
- 3-(((2,4-Dichlorobenzyl)sulfinyl)methyl)-4-methyl-5-((4-methylbenzyl)thio)-4H-1,2,4-triazole (CAS 344272-10-2)
- Folic Acid Impurity 53 (CAS CATO-C4X-139553)
- 5-[3-(3,4-Dimethoxyphenyl)-1,2,4-oxadiazol-5-yl]-3-(3,4,5-trimethoxyphenyl)-1,2,4-oxadiazole (CAS 1775439-98-9)
- Vericiguat Impurity 12 (CAS CATO-C4X-289612)
- Entacapone Impurity 63 (CAS CATO-C4X-162963)
- Propofol Impurity 22 (CAS 1988-10-9)
- Indobufen Impurity 67 (CAS CATO-C4X-179267)
- (R)-2-Amino-2-(2-hydroxyphenyl)acetic acid Hydrochloride (CAS 2829292-56-8)
- 2-(Aminomethyl)-3-chloro-5-(difluoromethyl)-4-methylpyridine (CAS 1807067-65-7)
- (4S,4'S)-2,2'-Cyclohexylidenebis[4,5-dihydro-4-isopropyloxazole] (CAS 1373357-00-6)
- Naloxone Impurity 19 (CAS CATO-C4X-149319)
- O-Benzyl-D-serine hydrochloride (CAS 1233494-98-8)
- 5-amino-1,3-dimethyl-2,3-dihydro-1H-1,3-benzodiazol-2-one (CAS 53439-88-6)
- Anorexigenic Peptide (CAS 69275-10-1)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com