Folic Acid Impurity 29 CAS 708-75-8

Description

Compared with other compounds in its class, the molecular weight of 177.16 g/mol gives Folic Acid Impurity 29 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

Folic Acid Impurity 29 (CAS 708-75-8) is a certified pharmaceutical impurity reference standard with the molecular formula C7H7N5O and a molecular weight of 177.16 g/mol.

The molecular framework of Folic Acid Impurity 29 (C7H7N5O) is characterized by an amide group, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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