Methotrexate EP Impurity E HCl CAS 70844-48-3
| CAS No. | 70844-48-3 |
| Molecular Formula | 2*C15H15N7O2 |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The occurrence of Methotrexate EP Impurity E HCl is closely related to a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
Methotrexate EP Impurity E HCl (CAS 70844-48-3) is a certified pharmaceutical impurity reference standard with the molecular formula C15H15N7O2HCl and a molecular weight of 361.79 g/mol.
Structurally, Methotrexate EP Impurity E HCl (C15H15N7O2HCl) contains a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. The molecule exhibits a degree of unsaturation (DBE) of 11.0, corresponding to approximately 11 ring(s) or double-bond equivalent(s). With a molecular weight of 361.79 g/mol, it falls into the moderately-high-molecular-weight range. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
CATO manufactures Methotrexate EP Impurity E HCl under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | 2*C15H15N7O2 HCl |
| Molecular Weight | 361.79 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 11.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1977 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 7 |
Related Methotrexate Standards
- Methotrexate Impurity 41 (CAS 101810-76-8)
- Methotrexate Dihydrate (CAS 1212284-68-8)
- Methotrexate Hydrate EP Impurity B (CAS 1217459-90-9)
- Methotrexate Hydrate (CAS 133073-73-1)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity E (CAS 19741-14-1)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate EP Impurity G (CAS 2519848-42-9)
Recommended Standards
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- 2-Undecenoic Acid (CAS 4189-02-0)
- 2-Methyl-3-(methylthio)-4,5-diphenylfuran (CAS 88319-95-3)
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- 1-(5-Chloro-1,2-benzoxazol-3-yl)methanamine hydrobromide (CAS 2097936-28-0)
- semaglutide (CAS 782487-28-9)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com