Methotrexate Impurity 71 CAS CATO-C4X-115471
| CAS No. | CATO-C4X-115471 |
| Molecular Formula | C17H19ClN2O8 |
| Molecular Weight | 414.79 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including Methotrexate Impurity 71.
Methotrexate Impurity 71 (CAS CATO-C4X-115471) is a certified pharmaceutical impurity reference standard with the molecular formula C17H19ClN2O8 and a molecular weight of 414.79 g/mol.
Based on its molecular formula C17H19ClN2O8, Methotrexate Impurity 71 contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 414.79 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C17H19ClN2O8 |
| Molecular Weight | 414.79 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor) |
| Purity | ≥95% |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 2 |
Related Methotrexate Standards
- Methotrexate Impurity 69 (CAS CATO-C4X-115469)
- Methotrexate Impurity 35 (CAS CATO-C4X-115435)
- Methotrexate Impurity 78 (CAS CATO-C4X-115478)
- Methotrexate impurity 55 (CAS CATO-C4X-115455)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity B (CAS 54-62-6)
- Methotrexate Impurity 2 (CAS 80407-56-3)
- Methotrexate EP Impurity F (CAS 51865-79-3)
Recommended Standards
- 3-Chlorosulfonyl-4-(2-methoxy-phenyl)-5-trifluoro-methyl-thiophene-2-carboxylic acid methyl ester (CAS 1431555-14-4)
- 6-((4-Chlorophenyl)sulfonyl)-2-phenyl-5-(trifluoromethyl)pyridazin-3(2H)-one (CAS 866131-12-6)
- N-[2-chloro-5-(trifluoromethyl)phenyl]-N'-[[2-(4-morpholinyl)-3-pyridinyl]methyl]-Urea (CAS 1211298-93-9)
- 2-(3-Chloro-5-(trifluoromethyl)pyridin-2-yl)-4-(2,4-dimethoxyphenyl)-5-methyl-2,4-dihydro-3H-1,2,4-triazol-3-one (CAS 861206-40-8)
- Levofloxacin impurity 90 (CAS CATO-C4X-137190)
- Diflorasone impurity 3 (CAS CATO-C4X-24803)
- Azilsartan Impurity 163 (CAS CATO-C4X-1096163)
- 2-Cyclopropyl-5-ethynyl-6-fluorobenzo[d]thiazole (CAS 2761212-91-1)
- 1-Formyl-4-(4-fluorophenyl)thiosemicarbazide (CAS 457609-53-9)
- methyl 2-chloro-5-((2,6-dichloropyrimidin-4-yl)amino)benzoate (CAS 1394347-61-5)
- N-(3-methylbenzyl)-2-(piperidin-4-yl)acetamide hydrochloride (CAS 1257851-02-7)
- 4-Formyl-N,N-dimethylbenzenesulfonamide (CAS 54049-92-2)
- 4-(3-(4-(Benzyloxy)phenoxy)propoxy)benzaldehyde (CAS 937602-07-8)
- Tirzepatide Impurity 47 (CAS CATO-C4X-324047)
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