Azathioprine Impurity N4 CAS 5167-18-0
| CAS No. | 5167-18-0 |
| Molecular Formula | C6H6N4O |
| Molecular Weight | 150.14 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Azathioprine Impurity N4 (CAS 5167-18-0) is a certified pharmaceutical impurity reference standard with the molecular formula C6H6N4O and a molecular weight of 150.14 g/mol.
The molecular framework of Azathioprine Impurity N4 (C6H6N4O) is characterized by an amide group, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
Physical & Chemical Properties
| Molecular Formula | C6H6N4O |
| Molecular Weight | 150.14 g/mol |
| Appearance | Off-white to pale yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1958 |
| Nitrogen Atoms | 4 |
Related Azathioprine Standards
- Azathioprine Impurity 12 (CAS 1192-53-6)
- Azathioprine Impurity 21 (CAS 30467-02-8)
- Azathioprine EP Impurity B (CAS 157930-13-7)
- Azathioprine EP Impurity B (CAS 50-44-2)
- Azathioprine Impurity 9 (CAS 1190008-04-8)
- Azathioprine EP Impurity A (CAS 4531-54-8)
- Azathioprine EP Impurity D (CAS 6339-54-4)
- Azathioprine EP Impurity C (CAS 4897-25-0)
Recommended Standards
- Tofacitinib Impurity 180 (CAS 100047-45-8)
- 2-(5-Fluoro-6-methylpyridin-2-yl)acetonitrile (CAS 1000555-87-2)
- 6-Methyl-1,7-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (CAS 30129-57-8)
- 2-(6-Fluoro-5-methylpyridin-3-yl)acetonitrile (CAS 1000556-88-6)
- Famotidine Impurity 02 (CAS 81152-53-6)
- Roflumilast Impurity 57 (CAS CATO-C4X-108457)
- Avibactam Impurity 77 (CAS CATO-C4X-134877)
- 6-(4-Aminophenyl)-1,3-diazinane-2,4-dione (CAS 90840-43-0)
- 1-(ethylsulfanyl)propan-2-amine (CAS 331826-46-1)
- tert-Butyl 6-Chloronicotinate (CAS 115309-57-4)
- N-(2,3-dichlorobenzyl)-N-isopropylglycine (CAS 1353963-90-2)
- 4-Benzyl-2-(2h-1,2,3,4-tetrazol-5-yl)morpholine (CAS 1269151-45-2)
- 2-[3-(difluoromethyl)-1h,4h,5h,6h-cyclopenta[c]pyrazol-1-yl]acetic acid (CAS 937599-56-9)
- Semaglutide impurity 271 (CAS CATO-C4X-2226271)
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