Methotrexate Impurity 65 CAS CATO-C4X-115465
| CAS No. | CATO-C4X-115465 |
| Molecular Formula | C28H27N9O6 |
| Molecular Weight | N/A |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
CATO supplies Methotrexate Impurity 65 (CAS CATO-C4X-115465), a pharmacopoeial impurity reference standard (molecular formula C28H27N9O6Na, MW 608.57 g/mol) used for the identification and quantification of trace impurities in APIs.
Based on its molecular formula C28H27N9O6Na, Methotrexate Impurity 65 contains a steroid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 608.57 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard supports related-substances testing and method development in line with ICH Q3A/Q3B guidance on impurity control, and is suitable for system suitability testing and forced-degradation studies.
As a certified reference material (CRM), Methotrexate Impurity 65 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C28H27N9O6 2*Na |
| Molecular Weight | 608.57 g/mol |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 20.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| Nitrogen Atoms | 9 |
Related Methotrexate Standards
- Methotrexate Impurity 41 (CAS 101810-76-8)
- Methotrexate Dihydrate (CAS 1212284-68-8)
- Methotrexate Hydrate EP Impurity B (CAS 1217459-90-9)
- Methotrexate Hydrate (CAS 133073-73-1)
- Methotrexate Impurity 11 (CAS 143873-72-7)
- Methotrexate EP Impurity E (CAS 19741-14-1)
- Methotrexate EP Impurity C (CAS 2410-93-7)
- Methotrexate EP Impurity G (CAS 2519848-42-9)
Recommended Standards
- Salcaprozate Impurity 10 (CAS CATO-C4X-250710)
- Semaglutide Impurity 122 (CAS CATO-C4X-2226122)
- Valsartan Impurity 150 (CAS CATO-C4X-1057150)
- (5-Oxaspiro[2.5]octan-1-yl)methanol (CAS 1514099-23-0)
- 1,3-Dichloro-5-(2-chloroethyl)benzene (CAS 1260784-43-7)
- (3-Chloro-5-(trifluoromethyl)pyridin-2-yl)(2-fluorophenyl)methanone (CAS 339097-08-4)
- (2E)-3-(2H-1,3-Benzodioxol-5-yl)-1-{[1,1'-biphenyl]-4-yl}prop-2-en-1-one (CAS 42580-60-9)
- 6-(2-Methyl-1-piperidinyl)-3-pyridinylamine (CAS 926194-75-4)
- N-(bicyclo[2.2.1]Hept-5-en-2-ylmethyl)cyclohexanamine (CAS 1218026-05-1)
- [Glu]17-Semaglutide (CAS CATO-CPTD788604)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com